Pathologists now engage with physical glass slides viewed through an optical microscope on their desk. Digital imaging of tissue slides is part of the future of anatomic pathology, but, while improving logistics, and more importantly, enabling advanced analytical tools, including AI-based interpretation of newly available digital data, laborious and technically challenging preparation of glass slides prior to scanning is still part of the work-flow (and cost structure). Alternative approaches that can bypass conventional histological processing and its associated delays and expense would be useful; a number of technologies for rapid, ex-vivo, slide-free microscopy are in R&D phase and a few have already been deployed as research-use-only commercial instruments. These contenders to replace standard histology appear promising for such uses as real-time surgical guidance, tumor margin assessment, rapid on-site (biopsy) evaluation (ROSE), pre-clinical research, and potentially even for final tissue diagnosis, while accelerating the velocity of care and decreasing costs.
Join us as Dr. Levenson discusses a few of these methods, including multiphoton and confocal microscopy, light-sheet microscopy, optical coherence tomography and structured illumination, with a focus on MUSE (Microscopy with UV Surface Excitation), a novel approach that demonstrates a favorable combination of speed, simplicity, robustness, and with images whose quality can not only equal, but in fact surpass, that of conventional pathology slide scans.
About the Speaker:
Richard Levenson, MD, FCAP, is Professor and Vice Chair for Strategic Technologies, Department of Pathology and Laboratory Medicine, UC Davis, where he develops novel imaging technologies. Board-certified in Anatomic Pathology, he received his MD at University of Michigan and pathology training at Washington University. A faculty position at Duke was followed by appointment at Carnegie Mellon University. He subsequently joined Cambridge Research & Instrumentation (now part of PerkinElmer), becoming VP of Research before returning to academia. He has helped develop multispectral microscopy systems and software for molecular pathology and diagnostics, multispectral and three-dimensional small-animal imaging systems, optical dynamic contrast techniques, orientation-independent birefringence microscopy, multiplexed ion-beam imaging, and most recently, real-time slide-free microscopy. He serves on multiple review panels, is section editor for Archives of Pathology and on the editorial board of Laboratory Investigation. Regrettably, he also successfully taught pigeons histopathology and radiology. He is co-founder of MUSE Microscopy, Inc. and the recipient of the UC Davis Chancellor’s Innovator of the Year (2018) award.
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